Spain’s Rafael Nadal plays the ball during his Mexican Tennis Open round 2 match against Australia’s Nick Kyrgios, in Acapulco, Mexico, Wednesday, Feb. 27, 2019. (AP Photo/Rebecca Blackwell)ACAPULCO, Mexico — Rafael Nadal tumbled out of the Mexican Open on Wednesday night, squandering three match points in a 3-6, 7-6 (2), 7-6 (6) loss to Australia’s Nick Kyrgios.Playing his first event since losing to Novak Djokovic in late January in the Australian Open final, the top-seeded Nadal dropped the first two match points on Kyrgios’ serve and the last one on his own first serve.ADVERTISEMENT MOST READ View comments Tom Brady most dominant player in AFC championship history Japeth Aguilar wins 1st PBA Finals MVP award for Ginebra Carpio hits red carpet treatment for China Coast Guard PLAY LIST 02:14Carpio hits red carpet treatment for China Coast Guard02:56NCRPO pledges to donate P3.5 million to victims of Taal eruption00:56Heavy rain brings some relief in Australia02:37Calm moments allow Taal folks some respite03:23Negosyo sa Tagaytay City, bagsak sa pag-aalboroto ng Bulkang Taal01:13Christian Standhardinger wins PBA Best Player award Gretchen Barretto’s daughter Dominique graduates magna cum laude from California college Ginebra beats Meralco again to capture PBA Governors’ Cup title In women’s play, two-time Australian Open champion Victoria Azarenka routed Tatjana Maria 6-2, 6-1 to set up a quarterfinal against fifth-seeded Sofia Kenin. Kenin led 6-4, 4-1 when Katie Boulter retired.Third-seeded Donna Vekic advanced with a 6-4, 7-5 victory over Christina McHale; Bianca Andreescu topped fourth-seeded Mihaela Buzarnescu 6-2, 7-5; seventh-seeded Saisai Zheng edged Timea Babos 4-6, 6-4, 6-3; and eighth-seeded Johanna Konta beat Varvara Flink 6-4, 6-1.In the quarterfinals, Vekic will play Konta, and Zheng will meet Andreescu.Lesia Tsurenko, the women’s winner the last two years, is skipping the event.Sports Related Videospowered by AdSparcRead Next Will you be the first P16 Billion Powerball jackpot winner from the Philippines? Rogue cops marked as Gamboa’s targets in his appointment as PNP chief Nadine Lustre’s phone stolen in Brazil Don’t expect to puff away at Tokyo Olympics 2020 Don’t miss out on the latest news and information. LATEST STORIES Ginebra beats Meralco again to capture PBA Governors’ Cup title Eugenie Bouchard’s bid for Australian Open spot ends in qualifying Nadal won in Acapulco in 2005 and 2013. On Tuesday night, the second-ranked Spanish star opened play with a 6-3, 6-3 victory over Mischa Zverev.Kyrgios set up a quarterfinal match against Stan Wawrinka, a 7-6 (5), 6-4 winner over seventh-seeded Steve Johnson in the hardcourt event at The Princess Mundo Imperial.FEATURED STORIESSPORTSGolden State Warriors sign Lee to multiyear contract, bring back ChrissSPORTSCoronation night?SPORTSThirdy Ravena gets offers from Asia, Australian ball clubsThe top seed also fell in the women’s event, with Brazilian qualifier Beatriz Haddad Maia beating Sloane Stephens 6-3, 6-3 for her first victory in seven career matches against players ranked in the top 10. The fourth-ranked Stephens, the 2017 U.S. Open, was making her first appearance in the event since winning the 2016 title.“It was just a tough match, obviously she played well,” Stephens said. “A tough day. But I’m not too sad about it. I’m just going to go back and work some more, practice some more and get ready for Indian Wells.” Haddad Maia will face Wang Yafan in the quarterfinals. Wang led Monica Puig 4-1 when Puig retired because of an injury.In men’s play, second-seeded Alexander Zverev beat David Ferrer 7-6 (0), 6-1 in the late match. He will play fifth-seeded Alex de Minaur, a winner over Feliciano Lopez in a walkover.Third-seeded John Isner beat fellow American Sam Querrey 6-4, 6-4 to set up a quarterfinal match against eighth-seeded John Millman. Millman beat Peter Gojowczyk 6-0, 6-2.Cameron Norrie beat fourth-seeded Diego Schwartzman 7-6 (5), 4-6, 6-3. Norrie will face Mackenzie McDonald, a 6-7 (7), 7-5, 6-3 winner over sixth-seeded Frances Tiafoe.Defending champion Juan Martin del Potro is sidelined by a knee injury.ADVERTISEMENT
Experimental study on the effect of diet on fatty acid and stable isotope profiles of the squid Lolliguncula brevis
Fatty acid and stable isotope analyses have previously been used to investigate foraging patterns of fish, birds, marine mammals and most recently cephalopod species. To evaluate the application of these methods for dietary studies in squid, it is important to understand the degree to which fatty acid and stable isotope signatures of prey species are reflected in the squids’ tissue. Four groups of Lolliguncula brevis were fed on prey species with distinctly different fatty acid and stable isotope profiles over 30 consecutive days. One group of squid were fed fish for fifteen days, followed by crustaceans for a further fifteen days. A second and third group were fed exclusively on fish or crustaceans for thirty days. And a fourth group was fed on a mixture of fish and crustaceans for thirty days. Analysis of squid tissue showed that, after 10 days of feeding, fatty acid profiles of squid tended to reflect those of their prey. Squid that fed on a single prey type, i.e. fish or crustacean, showed only minor modifications in fatty acid proportions after the initial change and fatty acid profiles were clearly distinguishable between the two feeding groups. Shifts in fatty acid proportions towards respective prey profiles could clearly be observed in squid the diet of which was swapped after 15 days. Clear differences could also be seen in fatty acid profiles of squid feeding on a mixed diet with trends towards either fish or crustacean fatty acid signatures. Stable isotope signatures of squid tissues clearly distinguished between animals feeding on different diets and supported findings from fatty acid analysis, thus indicating both methods to be viable tools in feeding studies on squid species.