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While the mainstream ending will be released in 500 + screens in shlf1314, a serious,Franz Ferdinand’s ‘Take Me Out’, for an annual meeting devoted to gene therapy—a long-struggling field that has clawed its way back to respectability with a string of promising results in small clinical trials Now many believe the powerful new gene-editing technology known as CRISPR will add to gene therapy’s newfound momentum But is CRISPR really ready for prime time Science explores the promise—and peril—of the new technology How does CRISPR work Traditional gene therapy works via a relatively brute-force method of gene transfer A harmless virus or some other form of so-called vector ferries a good copy of a gene into cells that can compensate for a defective gene that is causing disease But CRISPR can fix the flawed gene directly by snipping out bad DNA and replacing it with the correct sequence In principle that should work much better than adding a new gene because it eliminates the risk that a foreign gene will land in the wrong place in a cell’s genome and turn on a cancer gene And a CRISPR-repaired gene will be under the control of that gene’s natural promoter so the cell won’t make too much or too little of its protein product What has CRISPR accomplished so far Researchers have published successes with using CRISPR to treat animals with an inherited liver disease and muscular dystrophy and there will be more such preclinical reports at this week’s annual meeting of the American Society of Gene and Cell Therapy (ASGCT) The buzz around CRISPR is growing This year’s meeting includes 93 abstracts on CRISPR (of 768 total) compared with only 33 last year What’s more investors are flocking to CRISPR Three startups Editas Medicine Intellia Therapeutics and CRISPR Therapeutics have already attracted hundreds of millions of dollars So why isn’t CRISPR ready for prime time CRISPR still has a long way to go before it can be used safely and effectively to repair—not just disrupt—genes in people That is particularly true for most diseases such as muscular dystrophy and cystic fibrosis which require correcting genes in a living person because if the cells were first removed and repaired then put back too few would survive And the need to treat cells inside the body means gene editing faces many of the same delivery challenges as gene transfer—researchers must devise efficient ways to get a working CRISPR into specific tissues in a person for example CRISPR also poses its own safety risks Most often mentioned is that the Cas9 enzyme that CRISPR uses to cleave DNA at a specific location could also make cuts where it’s not intended to potentially causing cancer With these caveats do you even need CRISPR Conventional gene addition treatments for some diseases are so far along that it may not make sense to start over with CRISPR In Europe where one gene therapy is already approved for use for a rare metabolic disorder regulators are poised to approve a second for an immune disorder known as adenosine deaminase–severe combined immunodeficiency (SCID) And in the United States a company this year expects to seek approval for a gene transfer treatment for a childhood blindness disease called Leber congenital amaurosis (LCA) At the ASCGT meeting researchers working with the company Bluebird Bio will present interim data for a late-stage trial showing that gene addition can halt the progression of cerebral adrenoleukodystrophy a devastating childhood neurological disease Final results could help pave the way for regulatory approval Bluebird will also report on trials using gene transfer for two blood disorders sickle cell disease and β-thalassemia bringing these treatments closer to the clinic Except for LCA in which gene-carrying viruses are injected directly into eyes these diseases are treated by removing bone marrow cells from patients adding a gene to the cells and reinfusing the cells back into the patient New safer viral vectors have reduced risks of leukemia seen in a few patients in some early trials for immunodeficiency diseases Researchers are seeing “excellent clinical responses” says Donald Kohn of the University of California Los Angeles Although Kohn and other researchers have used an older gene-editing tool known as zinc finger nucleases to repair defective genes causing sickle cell disease and a type of SCID in cells in a dish only a tiny fraction of immature blood cells needed for the therapy to work end up with the gene corrected—far below the fraction altered by now standard gene transfer methods One reason is because the primitive blood cells aren’t dividing much (more on this below) Because gene-editing methods such as CRISPR are so much less efficient than gene addition for several diseases “I don’t think there will be a strong rationale for switching to editing” says Luigi Naldini of the San Raffaele Telethon Institute for Gene Therapy in Milan Italy CRISPR also has other issues Using CRISPR to cut out part of a gene—not correct the sequence—is relatively easy to do In fact this strategy is already being tested with zinc finger nucleases in a clinical effort to stop HIV infection In this treatment the nucleases are used to knock out a gene for a receptor called CCR5 in blood cells that HIV uses to get into cells But when CRISPR is used to correct a gene using a strand of DNA that scientists supply to cells not just to snip out some DNA it doesn’t work very well That’s because the cells must edit the DNA using a process called homology-directed repair or HDR that is only active in dividing cells And unfortunately most cells in the body—liver neuron muscle eye blood stem cells—are not normally dividing For this reason “knocking out a gene is a lot simpler than knocking in a gene and correcting a mutation” says Cynthia Dunbar president-elect of ASGCT and a gene therapy researcher at the National Heart Lung and Blood Institute in Bethesda Maryland Researchers are working on ways to get around this limitation The genes for HDR are present in all cells and it’s a matter of turning them on perhaps by adding certain drugs to the cells says CRISPR researcher Feng Zhang of the Broad Institute in Cambridge Massachusetts Another avenue is to find alternatives to the Cas9 system that don’t rely on the HDR process Zhang says But the low rate of HDR in most cells is one reason why the first use of CRISPR in the clinic will likely involve disrupting genes not fixing them For example several labs have shown in mice that CRISPR can remove a portion of the defective gene that causes Duchenne muscular dystrophy so that the remaining portion will produce a functional albeit truncated protein Editas hopes to start a clinical trial next year to treat a form of LCA blindness by chopping out part of the defective gene One proposed gene-editing treatment for sickle cell disease would similarly snip out some DNA so that blood cells produce a fetal form of the oxygen-carrying protein hemoglobin And CRISPR still has big safety risks The most-discussed safety risk with CRISPR is that the Cas9 enzyme which is supposed to slice a specific DNA sequence will also make cuts in other parts of the genome that could result in mutations that raise cancer risk Researchers are moving quickly to make CRISPR more specific For example in January one lab described a tweak to Cas9 that dramatically reduces off-target effects And in April in Nature another team showed how to make the enzyme more efficient at swapping out single DNA bases But immediate off-target cuts aren’t the only worry Although it’s possible to deliver CRISPR’s components into cells in a dish as proteins or RNA so far researchers can usually only get it working in tissue inside the body by using a viral vector to deliver the DNA for Cas9 into cells This means that even after Cas9 has made the desired cuts cells will keep cranking it out “The enzyme will still hang around over 10 20 years” Zhang says That raises the chances that even a very specific Cas9 will still make off-target cuts and that the body will mount an immune response to the enzyme This may not truly be a problem Zhang suggests His team created a mouse strain that is born with the gene for Cas9 turned on all the time so it expresses the enzyme in all cells for the animal’s entire life Even after interbreeding these mice for about 20 generations the mice “seem to be fine” with no obvious abnormal health effects Zhang says All the same “the most ideal case is we want to shut off the enzyme” And that may mean finding nonviral methods for getting Cas9 into cells such as ferrying the protein with lipids or nanoparticles—delivery methods that biologists have long struggled to make work in living animals Other long-standing obstacles to gene therapy will confront efforts using CRISPR too Depending on the disease any gene-edited cells may eventually die and patients could have to be treated multiple times Researchers using gene transfer and editing approaches are also both hindered by limits on how much DNA a viral vector can carry Right now CRISPR researchers often must use two different viruses to get CRISPR’s components into cells which is less efficient than a single vector So what’s the bottom line Gene therapists remain excited by CRISPR in part because it could tackle many more inherited diseases than can be treated with gene transfer Among them are certain immune diseases where the amount of the repaired protein must be precisely controlled In other cases such as sickle cell disease patients won’t get completely well unless a defective protein is no longer made by their cells so just adding a gene isn’t enough “It opens up a lot of diseases to gene therapy because gene addition wasn’t going to work” Dunbar says After more than 2 decades of seeing their field through ups and downs veterans of the gene therapy field are wary of raising expectations about CRISPR for treating diseases “Whenever there’s a new technology there’s a huge amount of excitement and everybody thinks it will be ready tomorrow to cure patients” says gene therapy researcher Mark Kay of Stanford University in Palo Alto California “It’s going to take some time” There are also many unknowns, "We’re anxious for agencies not to spend their precious research funds on the OSTP directive. With reporting by Jocelyn Kaiser.

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